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- Title
Impact of Neuro-Behçet Disease Immunoglobulin G on Neuronal Apoptosis.
- Authors
Giriş, Murat; Bireller, Sinem; Küçükali, Cem İsmail; Hanağasi, Haşmet; Değirmencioğlu, Sevgin; Tüzün, Erdem
- Abstract
Introduction: Parenchymal neuro-Behçet disease (NBD) is encountered in 5%-15% of Behçet disease (BD) patients and is characterized by inflammation of the brainstem and diencephalon structures. Neuronal apoptosis has been shown to participate in neuronal cell loss. Anti-neuronal antibodies have been identified in NBD patients. However, the pathogenic properties of these antibodies have not been studied. Methods: To delineate the potential pathogenic activity of serum antibodies on neurons, pooled sera from seven NBD patients and seven healthy controls were divided into purified immunoglobulin G (IgG) and IgG-depleted serum fractions, and each fraction was administered to cultured SH-SY5Y neuroblastoma cells. Cell death was evaluated with a toxicity assay and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining. Moreover, expression levels of several apoptosis markers were evaluated with real time polymerase chain reaction (PCR). Results: Administration of NBD IgG to cultured SH-SY5Y cells induced significantly increased cell death and apoptosis compared with other treatments. NBD IgG also enhanced the mRNA expression levels of major apoptosis and cell survival pathway factors. Conclusion: Our results suggest that IgGs isolated from the sera of NBD patients have a neurotoxic activity that is presumably mediated by apoptotic mechanisms.
- Subjects
APOPTOSIS; BEHCET'S disease; IMMUNOGLOBULINS; NEUROLOGICAL disorders; NEUROTOXICOLOGY; POLYMERASE chain reaction; RNA; SYNDROMES
- Publication
Archives of Neuropsychiatry / Nöropsikiyatri Arşivi, 2017, Vol 54, Issue 1, p67
- ISSN
1300-0667
- Publication type
Article
- DOI
10.5152/npa.2016.19421