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- Title
木屑添加对刺芹侧耳菌丝共生细菌 多样性和群落结构的影响.
- Authors
许 欣; 柳 婷; 孔旭强; 陈剑秋; 陈利丁; 黄 胜; 孙淑静
- Abstract
Some fungi engage symbiosis with bacteria, which can effectively promote the metabolism and growth of fungi. The diversity and community structure of bacteria can reflect the growth and substrate utilization of fungi. In this study, we analyzed the effects of different sawdust dosages on the community structure and diversity of bacteria associated with the hyphae of Pleurotus eryngii using high-throughput sequencing technology (UST) based on PCR-amplified 16S rRNA V3-V4 fragments. The results showed that the high-quality sequences from five groups of myeclia samples were clustered into 25 phyla, 52 classes, 114 orders, 199 families, and 406 genera. Protcobacteria (35.0%-85.9%) and Firmicutes (6.5%-38.4%) were the most abundant bacterial phyla, while Acinetobacter (14.8%-7 1.6%) and Pseudomonas ( 1.7%-22.3%) were the dominant symbiotic genera. Compared with the mycelia grown on the complete culture medium, sawdust addition could increase the diversity of bacteria coexisting with P. eryngii mycelia, and change the community structure of 10 dominant phyla and 9 dominant genera. P. eryngii cultivated in substrate containing 5g sawdust had the fastest mycelium growth rate, thick mycelia, and neatly edges. Furthermore, judging from the abundance and diversity, Pseudomonas and Lactobacillus became the dominant genera, which were positively correlated with the mycelia growth vigour. Sawdust, as an important carbon source, could affect the growth and development of P. eryngii and the community structure and diversity of haeleria coexisting with mycelia. This study would lay a theoretical foundation for exploring the molecular mechanism of sawdust and mycelium symbiosis affecting the growth and development of P. eryngii.
- Subjects
BACTERIAL diversity; FUNGAL growth; FUNGAL metabolism; WOOD waste; PLEUROTUS; MYCELIUM
- Publication
Yingyong Shengtai Xuebao, 2020, Vol 31, Issue 10, p3547
- ISSN
1001-9332
- Publication type
Article
- DOI
10.13287/j.1001-9332.202010.036