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- Title
A PCR-RFLP marker distinguishing Ophiostoma clavigerum from morphologically similar Leptographium species associated with bark beetles.
- Authors
Sangwon Lee; Jae-Jin Kim; Fung, Simon; Breuil, Colette
- Abstract
Ophiostoma clavigerum, carried by Dendroctonus ponderosae and Dendroctonus jeffreyi, has morphological characteristics that are similar to other Ophiostoma and Leptographium species. The partial β-tubulin gene of 45 strains belonging to seven species was amplified by PCR and digested by the restriction enzyme HinfI. The specific restriction fragment length polymorphism obtained for O. clavigerum provided the means for its reliable identification. We are also reporting that O. clavigerum ascocarps have short necks; this fact has not been shown previously.
- Subjects
OPHIOSTOMA; LEPTOGRAPHIUM; TUBULINS; GENES; BARK beetles
- Publication
Canadian Journal of Botany, 2003, Vol 81, Issue 11, p1104
- ISSN
0008-4026
- Publication type
Article
- DOI
10.1139/B03-101