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- Title
Off-Line Analysis of Red Blood Cell Velocity in Renal Arterioles.
- Authors
De Vriese, An S.; Verbeuren, Tony J.; Vallez, Marie-Odile; Lameire, Norbert H.; De Buyzere, Marc; Vanhoutte, Paul M.
- Abstract
Videomicroscopic methods with off-line analysis of microcirculatory parameters by multifunctional computer-assisted image analysis systems have significant advantages for in vivo microvascular research. A limitation of these methods is, however, that red blood cell velocities (V[sub RBC] ) exceeding 2 mm/s cannot be measured using standard video framing rates. In the present study, a high-speed video camera, recording up to 600 frames per second, was incorporated in the set-up, and V[sub RBC] was measured off-line with the line-shift-diagram method. The aim of this study was to test the reproducibility and validity of the method using a high-speed video camera and to evaluate its applicability in vivo. V[sub RBC] were measured in arterioles of the split hydronephrotic kidney. The intra- and interindividual variability was small for V[sub RBC] below 40 mm/s. The validity of the method was tested using the mass conservation principle and found to be at least as good as that of the dual-slit photometric technique. The present approach extends the application of videomicroscopy coupled to image analysis systems to the analysis of high V[sub RBC] .Copyright © 2000 S. Karger AG, Basel
- Subjects
MICROCIRCULATION; MICROSCOPY; ERYTHROCYTES; RENAL artery; BLOOD flow measurement
- Publication
Journal of Vascular Research, 2000, Vol 37, Issue 1, p26
- ISSN
1018-1172
- Publication type
Article
- DOI
10.1159/000025710