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- Title
DJ-1 Can Replace FGF-2 for Long-Term Culture of Human Pluripotent Stem Cells in Defined Media and Feeder-Free Condition.
- Authors
Kim, Julee; Baek, Sangki; Hong, Yean Ju; de Paula, Michelle Novais; Jahan Prima, Musharrat; Oh, Yeon-Mok; Cha, Sun-Shin; Do, Jeong Tae; Jang, Yeon Jin; Choe, Han
- Abstract
Conventional human pluripotent stem cell (hPSC) cultures require high concentrations of expensive human fibroblast growth factor 2 (hFGF-2) for hPSC self-renewal and pluripotency in defined media for long-term culture. The thermal instability of the hFGF-2 mandates media change every day, which makes hPSC culture costly and cumbersome. Human DJ-1 (hDJ-1) can bind to and stimulate FGF receptor-1. In this study, for the first time, we have replaced hFGF-2 with hDJ-1 in the essential eight media and maintained the human embryonic stem cells (hESCs), H9, in the defined media at feeder-free condition. After more than ten passages, H9 in both groups still successfully maintained the typical hESC morphology and high protein levels of pluripotency markers, SSEA4, Tra1-60, Oct4, Nanog, and ALP. DNA microarray revealed that more than 97% of the 21,448 tested genes, including the pluripotency markers, Sox2, Nanog, Klf4, Lin28A, Lin28B, and Myc, have similar mRNA levels between the two groups. Karyotyping revealed no chromosome abnormalities in both groups. They also differentiated sufficiently into three germ layers by forming in vitro EBs and in vivo teratomas. There were some variations in the RT-qPCR assay of several pluripotency markers. The proliferation rates and the mitochondria of both groups were also different. Taken together, we conclude that hDJ-1 can replace hFGF-2 in maintaining the self-renewal and the pluripotency of hESCs in feeder-free conditions.
- Subjects
HUMAN stem cells; FIBROBLAST growth factor 2; HUMAN embryonic stem cells; PLURIPOTENT stem cells; THERMAL instability; EPIBLAST; SOX2 protein
- Publication
International Journal of Molecular Sciences, 2021, Vol 22, Issue 11, p5954
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms22115954