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- Title
CRISPR-Cas9: A Revolutionary Tool for Cancer Modelling.
- Authors
Torres-Ruiz, Raul; Rodriguez-Perales, Sandra
- Abstract
The cancer-modelling field is now experiencing a conversion with the recent emergence of the RNA-programmable CRISPR-Cas9 system, a flexible methodology to produce essentially any desired modification in the genome. Cancer is a multistep process that involves many genetic mutations and other genome rearrangements. Despite their importance, it is difficult to recapitulate the degree of genetic complexity found in patient tumors. The CRISPR-Cas9 system for genome editing has been proven as a robust technology that makes it possible to generate cellular and animal models that recapitulate those cooperative alterations rapidly and at low cost. In this review, we will discuss the innovative applications of the CRISPR-Cas9 system to generate new models, providing a new way to interrogate the development and progression of cancers.
- Subjects
CRISPRS; CANCER genetics; GENETIC engineering; DISEASE progression; XENOGRAFTS; ZINC-finger proteins; DOUBLE-strand polymers
- Publication
International Journal of Molecular Sciences, 2015, Vol 16, Issue 9, p22151
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms160922151