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- Title
Astaxanthin preparation by fermentation of esters from Haematococcus pluvialis algal extracts with Stenotrophomonas species.
- Authors
Dong, Hao; Li, Xuemin; Xue, Changhu; Mao, Xiangzhao
- Abstract
Natural astaxanthin (Ax) is an additive that is widely used because of its beneficial biochemical functions. However, the methods used to produce free Ax have drawbacks. Chemical saponification methods produce several by-products, and lipase-catalyzed hydrolysis methods are not cost effective. In this study, a bacterial strain of Stenotrophomonas sp. was selected to enzymatically catalyze the saponification of Ax esters to produce free all-trans-Ax. Through single-factor experiments and a Box-Behnken design, the optimal fermentation conditions were determined as follows: a seed culture age of 37.79 h, an inoculum concentration of 5.92%, and an initial broth pH of 6.80. Under these conditions, a fermentation curve was drawn, and the optimal fermentation time was shown to be 60 h. At 60 h, the degradation rate of the Ax esters was 98.08%, and the yield of free all-trans-Ax was 50.130 μg/mL. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:649-656, 2016
- Subjects
ASTAXANTHIN; CHLAMYDOMONADACEAE; STENOTROPHOMONAS maltophilia; FERMENTATION; HYDROLYSIS; LIPASES; ESTERASES; CATALYTIC activity
- Publication
Biotechnology Progress, 2016, Vol 32, Issue 3, p649
- ISSN
8756-7938
- Publication type
Article
- DOI
10.1002/btpr.2258