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- Title
Búsqueda de reacción cruzada entre un antígeno de 65 kDa de Candida albicans con sueros de pacientes con lepra lepromatosa nodular y lepra tuberculoide.
- Authors
Ramos, Alejandro Palma; Fabila Aguilar, Arely Nallely; Castrillón Rivera, Laura E.; Petriz, Gilberto Casillas; Padilla Desgarennes, María del Carmen; Guzmán, Roberto Arenas; Vega Memije, María Elisa
- Abstract
Background: Leprosy (Hansen disease) is described as chronically contagious disease produced by Mycobacterium leprae that attacks mainly nerves and skin, with neurological damage that causes sensitivity lost and paralysis. Its classification is based in two fundamental polar types: lepromatous and tuberculoid and unstable cases: indeterminate and borderline. This classification is based in clinical, immunological, histopathological and bacteriological features. In lepromatous leprosy, patients have higher specific antibodies titles against M. leprae antigens, but their cellular responses are weak. In contrast, the tuberculoid leprosy patients show potent cellular immunity but their humoral response is low. Objective: To determine cross reactivity between Candida albicans antigens and antibodies against lepromatous leprosy and tuberculoid leprosy. Material and method: Peptides from total extract of Candida albicans between 9 and 96 kDa were purified and were exposed to four sera from lepromatous leprosy patients and three sera of tuberculoid leprosy patients, using anti-IgM or anti IgG peroxidase conjugates. The cross reactivity was evaluated by Western blot and enzyme immunoassay (ELISA) techniques. Conclusion: The 65 kDa antigen from C. albicans was well recognized by IgM from patients with leprosy, mainly lepromatous leprosy which demonstrated have high antibodies titles by ELISA and intense recognition by Western blot. With these results we can propose the use of this antigen as an auxiliary diagnostic tool in lepromatous nodular leprosy and possibly in future to use it in therapy because this protein has properties as immune cellular response stimulator.
- Subjects
HANSEN'S disease; COMMUNICABLE diseases; MYCOBACTERIAL diseases; CANDIDA albicans; DISEASE complications
- Publication
Dermatología Revista Mexicana, 2009, Vol 53, Issue 3, p113
- ISSN
0185-4038
- Publication type
Article