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- Title
Production and characterization of a noncytotoxic deletion variant of theAspergillus fumigatusallergen Aspf1 displaying reduced IgE binding.
- Authors
Garciá-Ortega, Lucía; Lacadena, Javier; Villalba, Mayte; Rodríguez, Rosalía; Crespo, Jesús F.; Rodríguez, Julia; Pascual, Cristina; Olmo, Nieves; Oñaderra, Mercedes; del Pozo, Álvaro Martínez; Gavilanes, José G.
- Abstract
Aspergillus fumigatusis responsible for many allergic respiratory diseases, the most notable of which − due to its severity − is allergic bronchopulmonary aspergillosis. Aspf1 is a major allergen of this fungus: this 149-amino acid protein belongs to the ribotoxin family, whose best characterized member isα-sarcin (EC 3.1.27.10). The proteins of this group are cytotoxic ribonucleases that degrade a unique bond in ribosomal RNA impairing protein biosynthesis. Aspf1 and its deletion mutant Aspf1Δ(7–22) have been produced as recombinant proteins; the deleted region corresponds to an exposedβ-hairpin. The conformation of these two proteins has been studied by CD and fluorescence spectroscopy. Their enzymatic activity and cytotoxicity against human rhabdomyosarcoma cells was also measured and their allergenic properties have been studied by using 58 individual sera of patients sensitized toAspergillus. Aspf1Δ(7–22) lacks cytotoxicity and shows a remarkably reduced IgE reactivity. From these studies it can be concluded that the deletedβ-hairpin is involved in ribosome recognition and is a significant allergenic region.
- Subjects
ASPERGILLUS; IMMUNOGLOBULIN E; NF-kappa B; RESPIRATORY diseases; BRONCHIAL diseases; RNA; PROTEINS
- Publication
FEBS Journal, 2005, Vol 272, Issue 10, p2536
- ISSN
1742-464X
- Publication type
Article
- DOI
10.1111/j.1742-4658.2005.04674.x