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- Title
HISTOMORPHOLOGICAL CHANGES IN ORGANS USING AN EXPERIMENTAL MODEL OF BALB/C MICE, INFECTED WITH DIFFERENT E. COLI STRAINS.
- Authors
Aniulienė, Albina; Pamparienė, Ina; Juožiukynienė, Nomeda; Zymantienė, Judita; Oberauskas, Vaidas; Plančiūnienė, Rita
- Abstract
The aim of this work was to determine an effect of endotoxin of E. coli strain ATCC 35218 serotype O6:H31:K-, strain RRW-1 serotype O8:H24:K-, and strain K-12 MG165 serotype O12:H48:K- on histomorphological changes in the organs using an experimental model of BALB/c mice. The first group (n=3) was control group and 0.1 ml 0.85% NaCl solution was injected into the peritoneum. The second group of mice (n=12) was divided into the 4 subgroups (n=3) and 0.1 ml of strain E. coli RRW-1 serotype O8:H24:K- according to the dilutions 1:1; 1:10; 1:100 and 1:1000 were injected into the peritoneum. The third group of mice (n=12) was divided into the 4 subgroups (n=3) and 0.1 ml of strain E. coli ATCC 35218 serotype O6:H31:K- according to the dilutions 1:1; 1:10; 1:100 and 1:1000 were injected into the peritoneum. The fourth group of mice (n=12) was divided into the 4 subgroups (n=3) and 0.1 ml of strain E. coli K-12 MG165 serotype O12:H48:K- according to the dilutions 1:1; 1:10; 1:100 and 1:1000 were injected into the peritoneum. All mice of the third group were dead within 20 hours after injection of strain E. coli ATCC 35218 serotype O6:H31:K- dilution 1:1. All strains and serotypes of E. coli caused histomorphological changes in the mice organs. The most severe changes in the organs were caused by ATCC 35218 serotype O6:H31:K- dilutions 1:1, 1:10, similar changes were caused by E. coli RRW-1 serotype O8:H24:K-, dilutions 1:1 and 1:10. Dilution 1:1 of K-12 MG165 serotype O12:H48:K- caused mild lesions in some organs. Pathogenic E. coli strains caused blood circulatory disturbances: hyperaemia, thrombosis, haemorrhages; hepatosis and nephrosis with immune response reaction, hyperplasia of lymphoid organs (spleen, lymph nodes).
- Subjects
ANIMAL models in research; HISTOLOGY; ESCHERICHIA coli; SEROTYPES; PERITONEUM
- Publication
Veterinarija ir Zootechnika, 2018, Vol 076, Issue 98, p9
- ISSN
1392-2130
- Publication type
Article