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- Title
Carboligation Reactions Mediated by Benzoylformate Decarboxylase Immobilized on a Magnetic Solid Support.
- Authors
Tural, Bilsen; Tural, Servet; Demir, Ayhan S.
- Abstract
ABSTRACT In this study, magnetic nanoparticles (Fe3O4, magnetite) with immobilized metal affinity ligands (MSS) were prepared and characterized by X-ray diffraction (XRD), transmission (TEM), fourier transform infrared spectroscopy (FTIR), and vibrating-sample magnetometer (VSM) methods for purification and immobilization of the histidine-tagged recombinant benzoylformate decarboxylase (BFD). The MSS support was shown to be eligible for selective binding of HIS-tagged BFD by SDS-page analysis. Loading capacity of the MSS support was determined as 43.6 ± 1.1 mg/g. The regeneration ability for protein binding was also studied. An immobilized BFD was tested to catalyze benzoin condensation and representative cross acyloin reaction. Conversion and enantiomeric excess values were comparable with that of free enzyme . Chirality 25:415-421, 2013. © 2013 Wiley Periodicals, Inc.
- Subjects
BENZOYLFORMATE decarboxylase; CARBOLINES; MAGNETIC nanoparticles; MAGNETITE; X-ray diffraction; TRANSMISSION electron microscopy; FOURIER transform infrared spectroscopy
- Publication
Chirality, 2013, Vol 25, Issue 7, p415
- ISSN
0899-0042
- Publication type
Article
- DOI
10.1002/chir.22181