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- Title
Shoot organogenesis and somatic embryogenesis from leaf and shoot explants of Ochna integerrima (Lour).
- Authors
Ma, Guohua; Jinfeng Lü; A. T. da Silva, Jaime; Xinhua Zhang; Jietang Zhao
- Abstract
Ochna integerrima is a medicinal and ornamental plant in Southeastern Asia. It has been listed as a rare and endangered species in China. Here we studied the effects of plant growth regulators and their concentrations on the induction of somatic embryogenesis and shoot organogenesis from leaf and shoot explants of O. integerrima for the first time. Cytokinins played a crucial role in somatic embryogenesis and shoot organogenesis. Among them, a higher concentration of thidiazuron (10.0-15.0 μM TDZ) could induce both somatic embryogenesis and adventitious shoot formation whereas low concentrations of TDZ (5.0 μM) could only induce adventitious shoots. However, 6-benzyladenine (BA at 5-15 μM) could only induce adventitious shoots. Shoot explants induced more adventitious shoots and somatic embryos than leaf explants when cultured on medium with the same concentration (5-15 μM) of TDZ or 15 μM BA. Medium containing 0.5 μM α-naphthaleneacetic acid and 8 μM indole-3-butyric acid and 0.1% activated charcoal could induce adventitious roots within 1 month. An efficient mass propagation and regeneration system has been established.
- Subjects
CHINA; OCHNA integerrima; PLANT morphogenesis; SOMATIC embryogenesis; PLANT embryology; PLANT regulators; PLANT shoots; ORNAMENTAL plants; PLANT propagation; ENDANGERED plants
- Publication
Plant Cell, Tissue & Organ Culture, 2011, Vol 104, Issue 2, p157
- ISSN
0167-6857
- Publication type
Article
- DOI
10.1007/s11240-010-9812-7