We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The use of TROSY for detection and suppression of conformational exchange NMR line broadening in biological macromolecules.
- Authors
Pervushin, Konstantin
- Abstract
The interference between conformational exchange-induced time-dependent variations of chemical shifts in a pair of scalar coupled 1H and 15N spins is used to construct novel TROSY-type NMR experiments to suppress NMR signal loss in [15N,1H]-correlation spectra of a 14-mer DNA duplex free in solution and complexed with the Antp homeodomain. An analysis of double- and zero-quantum relaxation rates of base 1H–15N moieties showed that for certain residues the contribution of conformational exchange-induced transverse relaxation might represent a dominant relaxation mechanism, which, in turn, can be effectively suppressed by TROSY. The use of the new TROSY method for exchange-induced transverse relaxation optimization is illustrated with two new experiments, 2D h1JHN,h2JNN-quantitative [15N,1H]-TROSY to measure h1JHN and h2JNN scalar coupling constants across hydrogen bonds in nucleic acids, and 2D (h2JNN+h1JNH)-correlation-[15N,1H]-TROSY to correlate 1HN chemical shifts of bases with the chemical shifts of the tertiary 15N spins across hydrogen bonds using the sum of the trans-hydrogen bond coupling constants in nucleic acids.
- Subjects
NUCLEIC acids spectra; SUPRAMOLECULAR chemistry; COUPLING constants; MOLECULAR biology; BIOCONJUGATES; QUALITATIVE chemical analysis
- Publication
Journal of Biomolecular NMR, 2001, Vol 20, Issue 3, p275
- ISSN
0925-2738
- Publication type
Article
- DOI
10.1023/A:1011208109853