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- Title
Aspartate?112 is the selectivity filter of the human voltage-gated proton channel.
- Authors
Musset, Boris; Smith, Susan M. E.; Rajan, Sindhu; Morgan, Deri; Cherny, Vladimir V.; DeCoursey, Thomas E.
- Abstract
The ion selectivity of pumps and channels is central to their ability to perform a multitude of functions. Here we investigate the mechanism of the extraordinary selectivity of the human voltage-gated proton channel, HV1 (also known as HVCN1). This selectivity is essential to its ability to regulate reactive oxygen species production by leukocytes, histamine secretion by basophils, sperm capacitation, and airway pH. The most selective ion channel known, HV1 shows no detectable permeability to other ions. Opposing classes of selectivity mechanisms postulate that (1) a titratable amino acid residue in the permeation pathway imparts proton selectivity, or (2) water molecules 'frozen' in a narrow pore conduct protons while excluding other ions. Here we identify aspartate 112 as a crucial component of the selectivity filter of HV1. When a neutral amino acid replaced Asp?112, the mutant channel lost proton specificity and became anion-selective or did not conduct. Only the glutamate mutant remained proton-specific. Mutation of the nearby Asp?185 did not impair proton selectivity, indicating that Asp?112 has a unique role. Although histidine shuttles protons in other proteins, when histidine or lysine replaced Asp?112, the mutant channel was still anion-permeable. Evidently, the proton specificity of HV1 requires an acidic group at the selectivity filter.
- Subjects
IONS; REACTIVE oxygen species; PROTONS; LEUCOCYTES; BASOPHILS; ION channels; PERMEABILITY; AMINO acids
- Publication
Nature, 2011, Vol 480, Issue 7376, p273
- ISSN
0028-0836
- Publication type
Article
- DOI
10.1038/nature10557