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- Title
Imaging dermal blood flow through the intact rat skin with an optical clearing method.
- Authors
Dan Zhu; Jing Wang; Zhongwei Zhi; Xiang Wen; Qingming Luo
- Abstract
Noninvasive detection of skin microcirculation is very significant for clinical diagnosis and therapy of peripheral vascular disease. In this study, an optical clearing method enables a laser speckle contrast imaging (LSCI) technique to image dermal blood flow through intact rat skin. The optical clearing effect of rat skin in vitrocaused by a mixture of PEG-400 and Thiazone is evaluated by a commercially available spectrophotometer with an integrating sphere. A LSCI system is applied to image the dermal blood flow of in vivorat skin after topical treatment of the agents. The results show that the reduced scattering coefficient (630 nm)is down to 60 of the initial value after 40 minof topical treatment of the mixture on skin in vitro. In vivoexperimental results show that a 12-mintreatment of the mixture on the epidermis can make the skin transparent and dermal vessels can be observed, while treatment with pure PEG-400 cannot. The process of skin optical clearing lasts, and the skin can be recovered by treatment of saline solution. Because optical clearing decreases the temporal contrast, dermal blood flow can be obtained by the laser speckle contrast imaging technique.
- Subjects
BLOOD flow; SKIN blood-vessels; LABORATORY rats; SPECKLE interference; EPIDERMIS; TRANSPARENCY (Optics); MEDICAL imaging systems
- Publication
Journal of Biomedical Optics, 2010, Vol 15, Issue 2, p026008
- ISSN
1083-3668
- Publication type
Article
- DOI
10.1117/1.3369739