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- Title
Possible involvement of Ca<sup>2+</sup>-independent phospholipase A<sub>2</sub> in protease-activated receptor-2-mediated contraction of rat urinary bladder.
- Authors
Kubota, Yuko; Nakahara, Tsutomu; Mitani, Akiko; Maruko, Takeshi; Saito, Maki; Sakamoto, Kenji; Ishii, Kunio
- Abstract
Possible involvement of Ca2+-independent phospholipase A2 (iPLA2) was examined in protease-activated receptor-2 (PAR-2)-mediated contraction of the rat urinary bladder. Both PAR-2 activating peptide (PAR-2 AP; SLIGRL-NH2) and trypsin produced a concentration-dependent contractile response in the urinary bladder preparations. These contractions were significantly (p<0.01) attenuated by indomethacin (10 µM), an inhibitor of cyclooxygenase, or bromoenol lactone (BEL; 10 µM), an inhibitor of iPLA2. On the other hand, the contractile responses to bradykinin were not significantly affected by BEL, although they were reduced by indomethacin. Arachidonyltrifluoromethyl ketone (AACOCF3; 30 µM), an inhibitor of cytosolic Ca2+-dependent phospholipase A2, did not affect the trypsin- and bradykinin-induced contractions. Both indomethacin and BEL had no inhibitory effect on the prostaglandin E2-induced contractions. These results suggest that PAR-2 activators and bradykinin stimulate the release of prostaglandins and thereby contract the rat urinary bladder smooth muscles. The release of prostaglandins by PAR-2 activators seems to be partly mediated by the iPLA2.
- Subjects
PHOSPHOLIPASES; CALCIUM in the body; PROTEOLYTIC enzymes; URINARY organs; ENZYME activation; INDOMETHACIN; TRYPSIN; BRADYKININ
- Publication
Naunyn-Schmiedeberg's Archives of Pharmacology, 2003, Vol 367, Issue 6, p588
- ISSN
0028-1298
- Publication type
Article
- DOI
10.1007/s00210-003-0753-0