We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Alterations of Urine TGF-β1 and bFGF following Bladder Outlet Obstruction: A Predictor for Detrusor Contractibility?
- Authors
Benkang Shi; Yaofeng Zhu; Laudon, Vincent; Lingxia Ran; Yuqiang Liu; Zhishun Xu
- Abstract
Objectives: To investigate the alterations of urine transforming growth factor-β1 (TGF-β1) and basic fibroblast growth factor (bFGF) following bladder outlet obstruction (BOO) in rats and to determine their correlation with the impaired detrusor contractibility. Methods: Wistar rats were divided into control, 2-week BOO 6-week and BOO groups. Impaired detrusor contractibility was quantified by measuring detrusor contraction force (DCF) of detrusor strip stimulated by carbachol. The enzyme-linked immunosorbent assay method was used to determine the urine levels of the 2 factors. Correlation analysis was conducted between DCF and urine levels of the 2 factors to see if there was an association between them after BOO. Results: DCF was found to be significantly lower in the 6-week BOO group than in the 2-week BOO and control groups. There is no significant difference regarding urine TGF-β1 between the 2-week BOO and control groups (p > 0.05). Urine TGF-β1 level in the 6-week BOO group was significantly higher than in the 2-week BOO (p < 0.05) and control groups (p < 0.05). There existed a negative correlation between DCF and urine TGF-β1 (p < 0.05). Conclusions: In an animal model, our results have suggested the potential role of urine TGF-β1 as a noninvasive biomarker to predict detrusor contractibility after BOO. Copyright © 2009 S. Karger AG, Basel
- Subjects
URINE; GROWTH factors; FIBROBLAST growth factors; BLADDER obstruction; CONTROL groups; ANIMAL models in research
- Publication
Urologia Internationalis, 2009, Vol 82, Issue 1, p43
- ISSN
0042-1138
- Publication type
Article
- DOI
10.1159/000176024