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- Title
In Vitro Sensitivity of Transplantable Leukemias to Endogenous Granuloid (GCE, GI-2) and Lymphoid (T<sub>4</sub>, T<sub>4</sub>-1) Inhibitors of Proliferation.
- Authors
Balázs, A.; Gál, F.; Klupp, T.; Blazsek, I.
- Abstract
The effect on cell proliferation of crude granulocyte and thymocyte extracts (GCE, T4) and of their target-specific fractions (Gl-2, T4-1) was studied in cultures with transplantable subacute myeloid and lymphoid leukemia (ML, LL). In the dose rage studied (1-500 μg/ml) each factor reduced 3H-TdR incorporation into acid-insoluble DNA of bone marrow, thymus and spleen cells with ML or LL as a function of the dose, approximately linearly. Normal bone marrow proved to be less sensitive to GCE than the ML one: according to parallel line bioassay by a factor of μ = 0.56. The reactivity of LL spleen and thymus is also higher to medium T4-1 concentrations (50-200 μg/ml) than that of normal lymphoid populations. T4-1 inhibits 3H-TdR incorporation into the DNA of LL spleen cells sub-maximally in 90': this effect lasts for > 7 hours. Because of its more homogeneous cell composition and higher sensitivity, subacute myeloid leukemia is more suitable for screening endógenous granuloid inhibitors than are homologous normal cell cultures. Copyright © 1978 S. Karger AG, Basel
- Publication
Oncology, 1978, Vol 35, Issue 1, p8
- ISSN
0030-2414
- Publication type
Article
- DOI
10.1159/000225247