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- Title
Advances in tomography: probing the molecular architecture of cells.
- Authors
Fridman, Karen; Mader, Asaf; Zwerger, Monika; Elia, Natalie; Medalia, Ohad
- Abstract
Visualizing the dynamic molecular architecture of cells is instrumental for answering fundamental questions in cellular and structural biology. Although modern microscopy techniques, including fluorescence and conventional electron microscopy, have allowed us to gain insights into the molecular organization of cells, they are limited in their ability to visualize multicomponent complexes in their native environment. Cryo-electron tomography (cryo-ET) allows cells, and the macromolecular assemblies contained within, to be reconstructed in situ, at a resolution of 2-6 nm. By combining cryo-ET with super-resolution fluorescence microscopy approaches, it should be possible to localize proteins with high precision inside cells and so elucidate a more realistic view of cellular processes. Thus, cryo-ET may bridge the resolution gap between cellular and structural biology.
- Subjects
CYTOLOGICAL research; CELLS; TOMOGRAPHY; ELECTRON microscopy; FLUORESCENCE microscopy; RADIOACTIVITY
- Publication
Nature Reviews Molecular Cell Biology, 2012, Vol 13, Issue 11, p736
- ISSN
1471-0072
- Publication type
Article
- DOI
10.1038/nrm3453