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- Title
Cloning of White Gene of the Melon Fly, Zeugodacus cucurbitae (Coquillett) (Diptera: Tephritidae) and in vitro Restriction Analysis of Different Single Guide RNAs (sgRNAs).
- Authors
PRADHAN, SANJAY KUMAR; ASOKAN, R.; SHIVANNA, B.
- Abstract
Zeugodacus cucurbitae (Coquillett) is a major pest affecting cucurbit crops. CRISPR/Cas9 mediated mutagenesis of target genes in Z. cucurbitae by generating a series of frame-shift mutation will help in changing physiology and behaviour of the insect. White gene is an important eye pigmentation gene widely used as marker gene in Drosophila melanogaster. PCR and Cloning of Z. cucurbitae white gene (2051bp) was performed. Off-target minimized gRNAs were designed by using bioinformatics online software CHOPCHOP by giving white coding sequences as input. Efficiency of the designed sgRNA was confirmed by in vitro restriction assay.
- Publication
Mysore Journal of Agricultural Sciences, 2023, Vol 57, Issue 2, p317
- ISSN
0047-8539
- Publication type
Article