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- Title
Characteristics of root boron nutrition confer high boron efficiency in Brassica napus cultivars.
- Authors
Yang, Lu; Zhang, Quan; Dou, Jina; Li, Ling; Guo, Longfei; Shi, Lei; Xu, Fangsen
- Abstract
Background and aims: Brassica napus has high boron (B) demand, but significant genotype differences exist with respect to B deficiency. The aim of this research was to elucidate the relationship between the different sensitivities of Brassica napus cultivars to low B stress and the characteristics of B uptake and transport to characterise the regulation of B efficiency in Brassica napus. Methods: B-efficient and B-inefficient Brassica napus cultivars were used to compare the uptake and transport of B using the stable isotope B tracer and grafting experiments, as well as expression of B transporters by RT-PCR. Results: B-efficient cultivars have significant advantages with regard to B limitation. The B-efficient cultivar HZ showed less severe B deficiency symptoms and higher dry biomass than the B-inefficient cultivars LW and LB. Both the amount of total B and the B concentration and accumulation in the shoots and roots of B-efficient HZ were higher than those of B-inefficient cultivars. In B-inefficient LW, the amount of total B and the B that was transported into shoots was less than in the other three cultivars and the content and accumulation of total B and B in the roots of B-inefficient LB were the lowest among all of the cultivars. When the roots of B-efficient HZ were used as stocks, the grafted plants showed B-efficient characteristics, such as mild B deficiency symptoms, and higher dry biomass and B accumulation, regardless of whether they originated from B-efficient or B-inefficient cultivars. In contrast, the grafted plants with B-inefficient LW used as stocks were B-inefficient. The expressions of BnBOR1;1c, BnBOR1;2a and BnNIP5;1 were up-regulated in roots under low B stress compared with the normal B condition. However, there was no obvious difference in the expressions of the three genes or of four other BnBOR1s between B-efficient and B-inefficient cultivars in low or normal B environments. Conclusions: These results indicate that the B efficiency of Brassica napus is controlled primarily by roots, which allow more uptake and accumulation of B in B-efficient cultivars than B-inefficient cultivars in a low B environment. However the molecular mechanism regulating B efficiency in Brassica napus remains to be determined.
- Subjects
RUTABAGA; BRASSICA varieties; BORON content of plants; GENE expression in plants; BIOACCUMULATION
- Publication
Plant & Soil, 2013, Vol 371, Issue 1/2, p95
- ISSN
0032-079X
- Publication type
Article
- DOI
10.1007/s11104-013-1669-1