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- Title
Decellularized extracellular matrix microparticles seeded with bone marrow mesenchymal stromal cells for the treatment of full-thickness cutaneous wounds.
- Authors
Westman, Amanda M.; Goldstein, Rachel L.; Bradica, Gino; Goldman, Scott M.; Randolph, Mark A.; Gaut, Joseph P.; Vacanti, Joseph P.; Hoganson, David M.
- Abstract
Extracellular matrix materials mechanically dissociated into submillimeter particles have a larger surface area than sheet materials and enhanced cellular attachment. Decellularized porcine mesothelial extracellular matrix microparticles were seeded with bone marrow-derived mesenchymal stromal cells and cultured in a rotating bioreactor. The mesenchymal stromal cells attached and grew to confluency on the microparticles. The cell-seeded microparticles were then encapsulated in varying concentrations of fibrin glue, and the cells migrated rapidly off the microparticles. The combination of microparticles and mesenchymal stromal cells was then applied to a splinted full-thickness cutaneous in vivo wound model. There was evidence of increased cell infiltration and collagen deposition in mesenchymal stromal cells-treated wounds. Cell-seeded microparticles have potential as a cell delivery and paracrine therapy in impaired healing environments.
- Subjects
FIBRIN; MESENCHYMAL stem cells; EXTRACELLULAR matrix
- Publication
Journal of Biomaterials Applications, 2019, Vol 33, Issue 8, p1070
- ISSN
0885-3282
- Publication type
Article
- DOI
10.1177/0885328218824759