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- Title
玉米钙依赖蛋白激酶38(ZmCDPK38)的生物信息学及表达特性分析.
- Authors
李建平; 足木热木·吐尔逊; 常晓春; 郝晓燕; 陈果; 高升旗; 孙良斌; 黄全生
- Abstract
【Objective】 To understand the genetic and evolutionary relationship between ZmCDPK38 and homologous CDPKs from other plant species and to predict the function of ZmCDPK38 theoretically by comparing with homologous genes with close relatives and known functions and to determine the expression characteristics of ZmCDPK38 genes in different tissues and under different stress conditions.【Method】 The online tools and MEGA-X software were used for bioinformatic analysis. Gene expression analysis was performed by real-time PCR.【Result】 ZmCDPK38 shared highly identical amino acid sequences with ZmCDPK26 and other six CDPKs from other species, indicating their closest genetic relationship. ZmCDPK38 protein contained four classical conserved domains of CDPK family member, including Ser/Thr protein kinase domains. The quantitative PCR results indicated that the ZmCDPK38 gene was expressed in a tissue-specific manner, which showed high expression level in maize leaves and less mRNA accumulation in stem, root, ear and tassel. Meanwhile the up-regulated transcription was observed during the treatment of drought and salt stress. On the contrary, the expression level showed little difference in response to low and high temperature except an initially increasedmRNA accumulation at 3h and 6h time-point after low temperature treatment.【Conclusion】 ZmCDPK38 protein contains four classical conserved domains of CDPK family member indicating its protein kinase characters. And the transcriptional analysis demonstrated that the expression of ZmCDPK38 gene was induced in response to drought and salt stress, suggesting that it may play a role in the regulation of drought and salt tolerance.
- Publication
Xinjiang Agricultural Sciences, 2021, Vol 58, Issue 1, p49
- ISSN
1001-4330
- Publication type
Article
- DOI
10.6048/j.issn.1001-4330.2021.01.006