We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Polymerase Chain Reaction-Based Sex Identification in the Greater Roadrunner.
- Authors
Santamaria, Carlos A.; Kelley, Samuel; Schulz, Gerral G.; Ransom, Jr., Dean; Hurtado, Luis A.
- Abstract
We determined a polymerase chain reaction (PCR)-based method to identify the sex of greater roadrunners (Geococcyx californianus). We found that previously reported primer combinations used in other bird species did not work reliably in roadrunners. However, a novel primer combination (P2-1237L) allowed for consistent and reliable sex identification of roadrunners. Conducting several PCR replicates per sample helped to identify occasional preferential amplification of the Z band that could occur in females (the heterogametic sex; i.e., WZ). Identifying the sex of individuals will allow for examination of sex-associated differences in home range size and habitat use, population demographics, and parenting behavior, as well as for a better understanding of the social system of roadrunners.
- Subjects
SEXING of animals; BIRD populations; BIRD behavior; ANIMAL population density; ANIMAL species; ANIMAL behavior; POLYMERASE chain reaction; ROADRUNNER; ZOOLOGICAL surveys
- Publication
Journal of Wildlife Management, 2010, Vol 74, Issue 6, p1395
- ISSN
0022-541X
- Publication type
Article
- DOI
10.2193/2009-248