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- Title
IMMUNOPHENOTYPE OF THE CANINE MAMMARY TUMORS REGARDING CD44 AND CD24 IMMUNOMARKERS.
- Authors
Gal, A. F.; Miclăuş, V.; Taulescu, M.; Bolfă, P.; Tăbăran, F.; Cătoi, C.
- Abstract
The cancer stem cell theory indicates that cancers develop from a subset of malignant cells that have stem cell characteristics (i.e., CD44+/CD24-). The aim of the study was to investigate the expression of CD44 and CD24 biomarkers in diverse canine mammary tumors. The material was represented by canine mammary tumors that were presented to the Laboratory of Pathologic Anatomy from the Faculty of Veterinary Medicine Cluj-Napoca, Romania. The tissue samples (i.e., from the mammary neoplasms) were fixed in 10% neutral buffered formalin and processed by paraffin technique. Immunohistochemical staining methods for CD44 (Santa Cruz) and CD24 (Santa Cruz) were performed on formalin-fixed, paraffin-embedded tissue sections. The labeled Labelled Streptavidin Biotin method (LSAB+system-HRP, Dako) and Rat ABC Staining System (Santa Cruz) was used for immunomarkers detection. The CD44 and CD24 positive cells were counted in 5 high-power microscopic fields (400x). Eleven canine mammary tumors (i.e., simple and complex mammary tumors) presented in diverse canine breeds were assessed. In our paper, canine mammary tumors had CD44+/CD24+ (i.e., 63.60%) or CD44-/CD24+ (i.e., 36.36%) immunophenotypes. Nevertheless, no CD44+/CD24- (revealing cancer stem cells) mammary tumors were noticed in our report. However, statistically significant correlations were established between the CD44 and CD24 evaluated biomarkers.
- Subjects
ROMANIA; MAMMARY gland tumors; BIOMARKERS; GENE expression; DOGS; FORMALDEHYDE; CD44 antigen; CD24 antigen
- Publication
Annals of the Romanian Society for Cell Biology, 2012, Vol 17, Issue 1, p328
- ISSN
2067-3019
- Publication type
Article