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- Title
COMPARATIVE STUDY OF DIANTHUS GIGANTEUS SUBSP. BANATICUS LEAVES ANATOMY IN DIFFERENT GROWING CONDITIONS I. LIGHT MICROSCOPY ASPECTS.
- Authors
Jarda, Liliana; Cristea, Victoria; Crăciun, C.; Tripon, S.
- Abstract
The present paper deals with anatomical changes in Dianthus giganteus subsp. banaticus which appear 3 months after maintenance in in vitro classical and photoautotrophic cultures. In vitro classical culture was made with 0.3% saccharose, while the photoautotrophic one had 0% saccharose, but had the culture vessels covered with transparent foil and ″sun cap″ filter, which allows gases exchanges and does not allow the phytopathogenic agents to get inside. Both culture types were maintained in the same light and temperature conditions, in the vegetation room. The comparisons were made between leaves of the plants coming from both culture types and from wild plants. The results show that plants maintained in in vitro classical culture have different structural changes, but these changes are reversible. The use of photoautotrophic culture before plant acclimatization has the role to get ready plants for autotrophic nutrition, to develop the photosynthetic apparatus, which is less developed in plants from in vitro classical culture. The structural aspect of leaves coming from photoautotrophic culture is an intermediary phase between the other two culture types. There were done structural investigations for a whole view of the leaf mesophyll. Following light microscopy investigations, it was observed that there are many changes in the structure of the leaf blade in the three types of plant cultures.
- Subjects
PINKS (Plants); MICROSCOPY; SUCROSE; ACCLIMATIZATION; AUTOTROPHIC bacteria
- Publication
Annals of the Romanian Society for Cell Biology, 2011, Vol 16, Issue 1, p331
- ISSN
2067-3019
- Publication type
Article