We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
EXPRESSION PROFILE OF VEGF AND EGFR mRNA IN ESOPHAGEAL AND GASTRIC CANCERS.
- Authors
Angelescu, Cristina; Burada, F.; Ioana, M.; Angelescu, R.; Riza, Anca; Mixich, F.; Voinescu, Doina; Cruce, M.; Săftoiu, A.
- Abstract
Gastric cancer is one of the most commonly diagnosed malignancies worldwide and an important public health problem. The local growth, invasion and distant metastasis of tumors are highly dependent on the development of new blood vessels. We aimed to evaluate the gene expression profiles of VEGF and EGFR in human esophageal and gastric cancer and to determine the correlations between the expression levels and clinico-pathological parameters of the tumor. 33 paired samples (tumor and non-malignant adjacent tissue) were collected from patients investigated by upper gastrointestinal endoscopy at the Research Center in Gastroenterology and Hepatology Craiova. Total RNA was isolated from the paired specimens and reverse-transcribed into the complementary DNA. Quantitative Real-Time PCR with TaqMan specific probes for VEGF-A and EGFR was performed. The studied genes are expressed both in tumor and peritumoral tissue, with a tendency of VEGF overexpression in tumor. We have also found that VEGF or EGFR mRNA relative expression correlates with several clinico-pathological characteristics. Further studies on higher number of patients are required to determine the utility of angiogenesis gene expression as progression and prognostic markers in esophageal and gastric carcinomas.
- Subjects
STOMACH cancer; ESOPHAGEAL cancer; EPIDERMAL growth factor; VASCULAR endothelial growth factors; MESSENGER RNA; NEOVASCULARIZATION; ANTISENSE DNA; GENE expression
- Publication
Annals of the Romanian Society for Cell Biology, 2011, Vol 16, Issue 1, p228
- ISSN
2067-3019
- Publication type
Article