We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The Maize High-Lysine Mutant opaque7 Is Defective in an Acyl-CoA Synthetase-Like Protein.
- Authors
Miclaus, Mihai; Wu, Yongrui; Xu, Jian-Hong; Dooner, Hugo K.; Messing, Joachim
- Abstract
Maize (Zea mays) has a large class of seed mutants with opaque or nonvitreous endosperms that could improve the nutritional quality of our food supply. The phenotype of some of them appears to be linked to the improper formation of protein bodies (PBs) where zein storage proteins are deposited. Although a number of genes affecting endosperm vitreousness have been isolated, it has been difficult to clone (o7), mainly because of its low penetrance in many genetic backgrounds. The o7- reference (o7-ref) mutant arose spontaneously in a W22 inbred, but is poorly expressed in other lines. We report here the isolation of o7-reference o7 with a combination of map-based cloning and transposon tagging. We first identified an candidate gene by map-based cloning. o7 The putative allele has a 12-bp in-frame deletion of codons 350-353 in a 528-codon-long acyl-CoA synthetase-like gene (ACS). o7-ref We then confirmed this candidate gene by generating another mutant allele from a transposon-tagging experiment using the Activator/Dissociation (Ac/Ds) system in a W22 background. The second allele, isolated from ~1 million gametes, presented a 2-kb Ac/Ds Ds insertion that resembles the single component of McClintock's original element, at codon 496 of the Ds double-Ds Dissociation ACS gene. PBs exhibited striking membrane invaginations in the allele and a severe number reduction in the -insertion o7-ref Ds mutant, respectively. We propose a model in which the ACS enzyme plays a key role in membrane biogenesis, by taking part in protein acylation, and that altered PBs render the seed nonvitreous.
- Subjects
CORN; FOOD supply; GENES; PROTEINS; GAMETES
- Publication
Genetics, 2011, Vol 189, Issue 4, p1271
- ISSN
0016-6731
- Publication type
Article
- DOI
10.1534/genetics.111.133918