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- Title
一株克氏原螯虾雷氏普罗威登斯菌的分离鉴定与联合药敏试验.
- Authors
董靖; 张露珊; 张国栋; 刘永涛; 刘绍春; 杨移斌; 艾晓辉
- Abstract
To clarify the cause of death of Procambarus clarkii (P. clarkii) during May to June and bring out treatment protocols, a bacterial pathogen (L20190509) was isolated from P. clarkia with typical symptom. The isolated pathogen was characterized by physiological and biochemical assay, 16S rRNA sequencing and phylogenetic analysis. Then the sensitivity of the isolated pathogen was tested by agar diffusion method, the synergistic effect of antibiotics and natural compounds were determined by checkerboard assays. The results showed that the 50% lethal concentration of the isolate to healthy P. clarkii was 9.27×104 CFU·mL-1, which exhibited similar physiological and biochemical characteristics to Providencia rettgeri (P. rettgeri) and shared over 99% homologies with P. rettgeri. Thus, the isolate was determined to be a P. rettgeri strain. The sensitivity study showed that the isolate was susceptible to imipenem, cefotaxime and enrofloxacin; intermediate to florfenicol and doxycycline; resistant to other tested antibiotics. Moreover, florfenicol combined with magnolol had synergistic effect against L20190509. Taken together, L20190509 is a bacterial pathogen of farmed P. clarkii which exhibited multi-resistance to antibiotics, these findings help to further acknowledge the pathogens of P. clarkii in May and formulate scientific and effective treatments.
- Publication
Acta Agriculturae Zhejiangensis, 2022, Vol 34, Issue 1, p24
- ISSN
1004-1524
- Publication type
Article
- DOI
10.3969/j.issn.1004-1524.2022.01.04