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- Title
Investigation of Seizure-Susceptibility in a Drosophila melanogaster Model of Human Epilepsy with Optogenetic Stimulation.
- Authors
Saras, Arunesh; Wu, Veronica V.; Brawer, Harlan J.; Tanouye, Mark A.
- Abstract
We examined seizure-susceptibility in a Drosophila model of human epilepsy using optogenetic stimulation of ReaChR (red-activatable channelrhodopsin). Photostimulation of the seizure-sensitive mutant parabss1 causes behavioral paralysis that resembles paralysis caused by mechanical stimulation, in many aspects. Electrophysiology shows that photostimulation evokes abnormal seizure-like neuronal firing in parabss1 followed by a quiescent period resembling synaptic failure and apparently responsible for paralysis. The pattern of neuronal activity concludes with seizure-like activity just prior to recovery. We tentatively identify the mushroom body as one apparent locus of optogenetic seizure initiation. The α/β lobes may be primarily responsible for mushroom body seizure induction.
- Subjects
DROSOPHILA melanogaster; ANIMAL models in epilepsy research; DISEASE susceptibility; OPTOGENETICS; SODIUM channels; ELECTROPHYSIOLOGY
- Publication
Genetics, 2017, Vol 206, Issue 4, p1739
- ISSN
0016-6731
- Publication type
Article
- DOI
10.1534/genetics.116.194779