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- Title
一株乳突赤壳属蕨麻致病菌的鉴定及生物学特性.
- Authors
王鑫慈; 李军乔; 李晨芹; 田甜; 曲俊儒
- Abstract
In order to identify the pathogen causing the root rot of Potentilla anserina L. and its biological characteristics, the strain MY-BSX4-1 was as material, which isolated from the tuberous root of Potentilla anserina in the artificial planting base of Menyuan, Qinghai province. The strain MY-BSX4-1 was analyzed by the pathogenic detection, morphological observation, rDNA-ITS sequence analysis. The growth and spore production of the pathogen were analyzed under different temperatures, pH, light, carbon sources, nitrogen sources and culture media. The results showed that MY-BSX4-1 was identified as Thelonectria olida according to its morphological characteristics and rDNA-ITS sequence analysis. It was identified to be the pathogenic fungus of Potentilla anserina L. according to Koch's rule. The biological characteristics showed that the optimum temperature of strain MY-BSX4-1 was 25 ℃for mycelial growth and spore production. The light condition was beneficial to mycelium growth, but had no obvious effect on spore production. The mycelium could grow well at pH 4.0~12.0, which the optimum pH for mycelium growth was 6.0~9.0 and the optimum pH was 8.0 for spore production. In carbon sources, soluble starch was beneficial to mycelial growth, lactose was beneficial to spore production, while fructose was unfavorable to mycelial growth. In nitrogen sources, beef extract was beneficial to mycelial growth, while ammonium nitrate was unfavorable to mycelial growth. Potentilla anserina L juice medium (JM) and JM (sodium nitrate) were beneficial to mycelial growth and spore production. The lethal temperature of mycelium was 47 ℃ for 10 min.
- Subjects
QINGHAI Sheng (China); SODIUM nitrate; ROOT rots; AMMONIUM nitrate; SEQUENCE analysis; PATHOGENIC fungi; LACTOSE
- Publication
Journal of Agricultural Science & Technology (1008-0864), 2023, Vol 25, Issue 5, p87
- ISSN
1008-0864
- Publication type
Article
- DOI
10.13304/j.nykjdb.2022.0973