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- Title
Three- dimensional imaging of vascular development in the mouse epididymis.
- Authors
Soubeyrand, Christelle Damon; Bongiovanni, Antonino; Chorfa, Areski; Goubely, Chantal; Pardanaud, Nelly Luc; Fragner, Laurence Piboin; Vachias, Caroline; Bravard, Stephanie; Guiton, Rachel; Thomas, Jean- Leon; Saez, Fabrice; Kocer, Ayhan; Tardivel, Meryem; Drevet, Joël R.; Berger, Joelle Henry
- Abstract
Long considered an accessory tubule of the male reproductive system, the epididymis is proving to be a key determinant of male fertility. In addition to its secretory role in ensuring functional maturation and survival of spermatozoa, the epididymis has a complex immune function. Indeed, it must manage both peripheral tolerance to sperm antigens foreign to the immune system and the protection of spermatozoa as well as the organ itself against pathogens ascending the epididymal tubule. Although our knowledge of the immunobiology of this organ is beginning to accumulate at the molecular and cellular levels, the organization of blood and lymphatic networks of this tissue, important players in the immune response, remains largely unknown. In the present report, we have taken advantage of a VEGFR3:YFP transgenic mouse model. Using high- resolution three- dimensional (3D) imaging and organ clearing coupled with multiplex immunodetections of lymphatic (LYVE1, PDPN, PROX1) and/or blood (PLVAP/Meca32) markers, we provide a simultaneous deep 3D view of the lymphatic and blood epididymal vasculature in the mature adult mouse as well as during postnatal development.
- Subjects
EPIDIDYMIS; LYMPHOID tissue; SPERMATOZOA; MICE; TRANSGENIC mice; IMMUNE complexes
- Publication
eLife, 2023, p1
- ISSN
2050-084X
- Publication type
Article
- DOI
10.7554/eLife.82748