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- Title
Genes expressed in Japanese flounder Paralichthys olivaceus spleen: analysis of genes involved in immune function.
- Authors
Arma, Nur Rahmawaty; Hirono, Ikuo; Aoki, Takashi
- Abstract
Expressed sequence tag (EST) analysis is an efficient tool for gene discovery and for profiling gene expression. A cDNA library developed from messenger RNA of the Japanese flounder, Paralichthys olivaceus spleen was constructed by directional cloning, in order to isolate functional genes involved in immunity in fish. A total of 1010 ESTs from the library were sequenced and compared with sequences in the GenBank database. Of the 1010 ESTs, 618 ESTs (61%) were identified as being homologous with known genes from many organisms by BLAST searches, whereas 392 (39%) appeared to be unknown and are likely to represent newly described genes. Of the identified genes, 105 (17%) encoded proteins associated with cell/organism defence and homeostasis. Of these 105 genes, 21 were identified for the first time in Japanese flounder. These included macrophage inflammatory protein (MIP)-3 α, granulin-A, novel immune-type receptor (NITR), interferon regulatory factor (IRF)-10, presenilin-like protein-2, and antizyme inhibitor. A comparison of ESTs derived from spleen, kidney, liver and leukocytes suggested that expression of immune-related genes in different tissues, organs, or cells are different.
- Subjects
FLATFISHES; GENE expression; NUCLEOTIDE sequence; FISH genetics; PARALICHTHYS; FISH immunology; FISH physiology; FISHERY sciences
- Publication
Fisheries Science, 2005, Vol 71, Issue 6, p1304
- ISSN
0919-9268
- Publication type
Article
- DOI
10.1111/j.1444-2906.2005.01097.x