We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Cryopreservation diluents for spermatozoa of Sakhalin taimenHucho perryi.
- Authors
Kusuda, Satoshi; Koide, Nobuhisa; Kawamula, Hiloshi; Teranishi, Tetsuo; Nakajima, Jun-Ichiro; Yamaha, Etsuro; Arai, Katsutoshi; Ohta, Hiromi
- Abstract
To develop a suitable cryopreservation diluent for spermatozoa of the endangered Sakhalin taimenHucho perryi, all possible combinations of cryoprotectants (glycerol, dimethyl sulfoxide[DMSO], methanol) and extenders (fetal bovine serum[FBS], 300 mM glucose solution[GS], artificial seminal plasma for masu salmon) were examined by observing sperm motility 10 s after thawing. Spermatozoa cryopreserved with diluents such as mixtures of 10% glycerol plus 90% FBS, 10% DMSO plus 90% FBS, and 10% methanol plus 90% GS showed the highest motility. The maximal post-thaw motility was observed at 10% among all concentrations (0, 5, 10, 15 and 20%) of these three cryoprotectants. No significant difference among three diluents was observed in motility at 10 s. Mixtures of 10% glycerol plus 90% FBS, 10% DMSO plus 90% FBS, and 10% methanol plus 90% GS are suitable cryopreservation diluents for Sakhalin taimen spermatozoa.
- Subjects
CRYOPRESERVATION of organs, tissues, etc.; SPERMATOZOA; GAMETES; HUCHO; SALMONIDAE; OSTEICHTHYES
- Publication
Fisheries Science, 2005, Vol 71, Issue 2, p293
- ISSN
0919-9268
- Publication type
Article
- DOI
10.1111/j.1444-2906.2005.00963.x