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- Title
UFD2a mediates the proteasomal turnover of p73 without promoting p73 ubiquitination.
- Authors
Hosoda, Mitsuchika; Ozaki, Toshinori; Miyazaki, Kou; Hayashi, Syunji; Furuya, Kazushige; Watanabe, Ken-ichi; Nakagawa, Takahito; Hanamoto, Takayuki; Todo, Satoru; Nakagawara, Akira
- Abstract
p73 protein level is kept extremely low in mammalian cultured cells and its stability may be regulated by not only the ubiquitin/proteasome-dependent proteolysis but also through other unidentified mechanisms. Here, we found for the first time that p73 is physically as well as functionally associated with the U-box-type E3/E4 ubiquitin ligase UFD2a. The immunoprecipitation experiments demonstrated that this interaction is mediated by the COOH-terminal region of p73α containing SAM domain. During the cisplatin-induced apoptosis in SH-SY5Y neuroblastoma cells, p73α accumulated at a protein level, whereas the endogenous UFD2a was significantly reduced in response to cisplatin. Ectopic expression of UFD2a decreased the half-life of p73α in association with a significant inhibition of the p73α-mediated transactivation as well as proapoptotic activity. Downregulation of endogenous UFD2a by antisense strategy resulted in a remarkable accumulation of p73α. Unexpectedly, UFD2a-mediated degradation of p73α was sensitive to the proteasomal inhibitor, however, UFD2a did not affect the ubiquitination levels of p73α. Taken together, our present findings imply that UFD2a might promote the proteasomal turnover of p73 in a ubiquitination-independent manner, and also suggest that UFD2a might play an important role in the regulation of cisplatin-induced apoptosis mediated by p73.Oncogene (2005) 24, 7156–7169. doi:10.1038/sj.onc.1208872; published online 19 September 2005
- Subjects
PROTEINS; UBIQUITIN; CELLS; LIGASES; ENZYMES; CISPLATIN; ALKYLATING agents; APOPTOSIS
- Publication
Oncogene, 2005, Vol 24, Issue 48, p7156
- ISSN
0950-9232
- Publication type
Article
- DOI
10.1038/sj.onc.1208872