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- Title
<italic>Peromyscus leucopus</italic> mouse brain transcriptome response to Powassan virus infection.
- Authors
Mlera, Luwanika; Meade-White, Kimberly; Dahlstrom, Eric; Baur, Rachel; Kanakabandi, Kishore; Virtaneva, Kimmo; Porcella, Stephen F.; Bloom, Marshall E.
- Abstract
Powassan virus (POWV) is a tick-borne <italic>Flavivirus</italic> responsible for life-threatening encephalitis in North America and some regions of Russia. The ticks that have been reported to transmit the virus belong to the <italic>Ixodes</italic> species, and they feed on small-to-medium-sized mammals, such as <italic>Peromyscus leucopus</italic> mice, skunks, and woodchucks. We previously developed a <italic>P. leucopus</italic> mouse model of POWV infection, and the model is characterized by a lack of clinical signs of disease following intraperitoneal or intracranial inoculation. However, intracranial inoculation results in mild subclinical encephalitis from 5 days post infection (dpi), but the encephalitis resolves by 28 dpi. We used RNA sequencing to profile the <italic>P. leucopus</italic> mouse brain transcriptome at different time points after intracranial challenge with POWV. At 24 h post infection, 42 genes were significantly differentially expressed and the number peaked to 232 at 7 dpi before declining to 31 at 28 dpi. Using Ingenuity Pathway Analysis, we determined that the genes that were significantly expressed from 1 to 15 dpi were mainly associated with interferon signaling. As a result, many interferon-stimulated genes (ISGs) were upregulated. Some of the ISGs include an array of <italic>TRIMs</italic> (genes encoding tripartite motif proteins). These results will be useful for the identification of POWV restriction factors.
- Subjects
POWASSAN (Disease); ENCEPHALITIS; TICK-borne diseases; PEROMYSCUS leucopus; LABORATORY rats
- Publication
Journal of NeuroVirology, 2018, Vol 24, Issue 1, p75
- ISSN
1355-0284
- Publication type
Article
- DOI
10.1007/s13365-017-0596-y