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- Title
Investigation of rs121918356 and rs121918355 LTBP2 Mutations and LTBP2 Serum Levels in Primary Congenital Glaucoma in a Sample of Iraqi Children.
- Authors
Al-Rubaꞌei, Salwa H. N.; Jubair, Suzanne; Al-Sharifi, Ali N. M.; Al-Moosawi, Mohanned M. B.
- Abstract
Primary congenital glaucoma (PCG) is a severe type of glaucoma which occurs early in life and is a leading cause of blindness in early childhood. Latent Transforming beta Binding Protein 2 (LTBP2) gene is reported to be a PCG-related gene. This study is designed to investigate the involvement of LTBP2 mutations (rs121918356 and rs121918355) in the PCG incidence in a sample of Iraqi children, and determine the LTBP2 protein both in the patients and the control groups. Venous blood was collected from one-hundred child patients diagnosed with PCG and one-hundred healthy children. Genomic DNA was extracted, and two DNA fragments concerning the two mutations were amplified. The mutations were followed up using the restriction fragment length polymorphism technique and direct sequencing. Serum LTBP2 protein was measured using ELISA technique. No mutation was detected in any of the examined samples. A highly significant (p=0.0001) elevated serum LTBP2 protein was observed in the patients' group compared to the control group. In conclusion, LTBP2 mutations (rs121918356 and rs121918355) are not related to the PCG in the Iraqi population. The PCG patients have higher LTBP2 protein levels compared to the healthy controls.
- Subjects
RETROLENTAL fibroplasia; CHILDREN; CONGENITAL glaucoma
- Publication
Jordan Journal of Biological Sciences, 2019, Vol 12, Issue 1, p77
- ISSN
1995-6673
- Publication type
Article