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- Title
Fission Yeast CENP-C (Cnp3) Plays a Role in Restricting the Site of CENP-A Accumulation.
- Authors
Michiko Suma; Teppei Kitagawa; Yukiko Nakase; Norihiko Nakazawa; Mitsuhiro Yanagida; Tomohiro Matsumoto
- Abstract
The centromere is a chromosomal locus where a microtubule attachment site, termed kinetochore, is assembled in mitosis. In most eukaryotes, with the exception of holocentric species, each chromosome contains a single distinct centromere. A chromosome with an additional centromere undergoes successive rounds of anaphase bridge formation and breakage, or triggers a cell cycle arrest imposed by DNA damage and replication checkpoints. We report here a study in Schizosaccharomyces pombe to characterize a mutant (cnp3-1) in a gene encoding a homolog of mammalian centromere-specific protein, CENP-C. At the restrictive temperature 36, the Cnp3-1 mutant protein loses its localization at the centromere. In the cnp3-1 mutant, the level of the Cnp1 (a homolog of a centromere-specific histone CENP-A) also decreases at the centromere. Interestingly, the cnp3-1 mutant is prone to promiscuous accumulation of Cnp1 at non-centromeric regions, when Cnp1 is present in excess. Unlike the wild type protein, Cnp3-1 mutant protein is found at the sites of promiscuous accumulation of Cnp1, suggesting that Cnp3-1 may stabilize or promote accumulation of Cnp1 at non-centromeric regions. From these results, we infer the role of Cnp3 in restricting the site of accumulation of Cnp1 and thus to prevent formation of de novo centromeres.
- Subjects
EUKARYOTES; MICROTUBULES; SCHIZOSACCHAROMYCES pombe
- Publication
G3: Genes | Genomes | Genetics, 2018, Vol 8, Issue 8, p2723
- ISSN
2160-1836
- Publication type
Article
- DOI
10.1534/g3.118.200486