We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Cytochalasin-B-Inducible Nanovesicle Mimics of Natural Extracellular Vesicles That Are Capable of Nucleic Acid Transfer.
- Authors
Oshchepkova, Anastasiya; Neumestova, Alexandra; Matveeva, Vera; Artemyeva, Lyudmila; Morozova, Ksenia; Kiseleva, Elena; Zenkova, Marina; Vlassov, Valentin
- Abstract
Extracellular vesicles provide cell-to-cell communication and have great potential for use as therapeutic carriers. This study was aimed at the development of an extracellular vesicle-based system for nucleic acid delivery. Three types of nanovesicles were assayed as oligonucleotide carriers: Mesenchymal stem cell-derived extracellular vesicles and mimics prepared either by cell treatment with cytochalasin B or by vesicle generation from plasma membrane. Nanovesicles were loaded with a DNA oligonucleotide by freezing/thawing, sonication, or permeabilization with saponin. Oligonucleotide delivery was assayed using HEK293 cells. Extracellular vesicles and mimics were characterized by a similar oligonucleotide loading level but different efficiency of oligonucleotide delivery. Cytochalasin-B-inducible nanovesicles exhibited the highest level of oligonucleotide accumulation in HEK293 cells and a loading capacity of 0.44 ± 0.05 pmol/µg. The loaded oligonucleotide was mostly protected from nuclease action.
- Subjects
OLIGONUCLEOTIDES; NUCLEIC acids; CELL membranes
- Publication
Micromachines, 2019, Vol 10, Issue 11, p750
- ISSN
2072-666X
- Publication type
Article
- DOI
10.3390/mi10110750