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- Title
RNA Probes for Visualization of Sarcin/ricin Loop Depurination without Background Fluorescence.
- Authors
Klimek, Robin; Kaiser, Christoph; Murmann, Nina S.; Kaltenschnee, Nina; Spanò, Teresa; Wachtveitl, Josef; Schuman, Erin M.; Heckel, Alexander
- Abstract
Protein synthesis via ribosomes is a fundamental process in all known living organisms. However, it can be completely stalled by removing a single nucleobase (depurination) at the sarcin/ricin loop of the ribosomal RNA. In this work, we describe the preparation and optimization process of a fluorescent probe that can be used to visualize depurination. Starting from a fluorescent thiophene nucleobase analog, various RNA probes that fluoresce exclusively in the presence of a depurinated sarcin/ricin‐loop RNA were designed and characterized. The main challenge in this process was to obtain a high fluorescence signal in the hybridized state with an abasic RNA strand, while keeping the background fluorescence low. With our new RNA probes, the fluorescence intensity and lifetime can be used for efficient monitoring of depurinated RNA.
- Subjects
RICIN; FLUORESCENCE; RNA; RIBOSOMAL RNA; FLUORESCENT probes; PROTEIN synthesis
- Publication
Chemistry - An Asian Journal, 2022, Vol 17, Issue 24, p1
- ISSN
1861-4728
- Publication type
Article
- DOI
10.1002/asia.202201077