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- Title
Antimicrobial Effect of Bee Collected Pollen Extract to Enterobacteriaceae Genera after Application of Bee Collected Pollen in their Feeding.
- Authors
Hleba, Lukáš; Pochop, Jaroslav; Felšöciová, Soňa; Petrová, Jana; Čuboň, Juraj; Pavelková, Adriana; Kačániová, Miroslava
- Abstract
In this study we researched antimicrobial activity of bee pollen extracts to Enterobacteriaceae genera isolated from chicken intestinal tract after application of bee collected pollen in their feeding. We used well plate agar diffusion method for antimicrobial testing of bee pollen extract and disc diffusion method for antibiotic susceptibility testing of bacteria by EUCAST. Identification of bacteria was done by test kit Enterotest 24. We identified tree bacterial strains: E. coli, P. mirabilis and K. oxytoca. We determined that K. oxytoca was resistant to ampicillin only and others identified strain were sensitive to used antibiotics. Also we determined antimicrobial effect of bee pollen extract to all tested strains of Enterobacteriaceae genera which were isolated from intestinal tract of chicken after application of bee collected pollen extract in their feeding. From obtained results we could be conclude that bacteria isolated from chicken after application of bee pollen extract had more resistance to bee collected pollen extract in in vitro experiment as E. coli CCM 3988, which did not be in contact with bee pollen extract.
- Subjects
ANTI-infective agents; FOODBORNE diseases; ENTEROBACTERIACEAE; POLLEN extract; ESCHERICHIA coli
- Publication
Scientific Papers: Animal Science & Biotechnologies / Lucrari Stiintifice: Zootehnie si Biotehnologii, 2013, Vol 46, Issue 2, p108
- ISSN
1841-9364
- Publication type
Article