We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Antidiabetic, Antihyperlipidemic, Antioxidant Effects and Regulation of miRNA Expression by Dianthus orientalis Adams Extract in Diabetic rat Model.
- Authors
Ahmed, Vian Abubaker; Rahman, Heshu Sulaiman; Mohammed Raheem, Mohammed Omer; Othman, Hemn Hassan; Algarawi, Maha; Ibnaouf, Khalid Hassan
- Abstract
Background: The Mediterranean area is a diversity center for the genus Dianthus. However, species belonging to this genus, including Dianthus orientalis Adams (DOA), have not been investigated for their medicinal activities. Objectives: To investigate antidiabetic, antihyperlipidemic, antioxidant effects and molecular mechanism of D. orientalis Adams leaf extract (DOAE) in an animal model. Materials and methods: The plant leaves were collected from July to August 2021 from Penjween district, Sulaimaniyah, Iraq and then identified, authenticated, shadow-dried, and extracted using pure methanol. Thirty rats were divided randomly into 5 groups of 6 animals each. Group 1 was control negative (CN) and received distilled water (DW). Group 2 was diabetic control (DC) that received DW, while group 3 was control positive (CP) treated with Glibenclamide (GLB, 0.6 mg/kg body weight). Groups 4 and 5 were diabetic rats who received a low-dose (30 mg/kg) and a high-dose (90 mg/kg) of DOAE orally for 4 weeks. Then, lipid profile, total antioxidant capacity, histopathological examinations, and molecular studies were conducted. Results: DOAE was more effective than GLB in reducing blood glucose, lipid parameters, liver enzymes, and renal function. Micromorphological assay of livers, kidneys, and pancreas revealed significant restoration in diabetic groups treated with DOAE (90 mg/kg) and GLB compared to the DC group. Microribonuclic acid-21 (miR-21) was significantly expressed in DC but markedly lowered in both DOAE groups, while miR-24 and miR-126 were significantly suppressed in DC and expressed in the DOAE-treated groups. Conclusions: DOAE exerted significant antihyperglycemic, anti-dyslipidemic, antioxidant, and hepatorenal protective effects in diabetic rats.
- Subjects
IRAQ; GENE expression; LABORATORY rats; RNA regulation; ANIMAL disease models; OXIDANT status
- Publication
Natural Product Communications, 2024, Vol 19, Issue 6, p1
- ISSN
1934-578X
- Publication type
Article
- DOI
10.1177/1934578X241259377