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- Title
Pathogen-nematode interaction: Nitrogen supply of Listeria monocytogenes during growth in Caenorhabditis elegans.
- Authors
Kern, Tanja; Kutzner, Erika; Eisenreich, Wolfgang; Fuchs, Thilo M.
- Abstract
L isteria monocytogenes is a Gram-positive facultatively intracellular human pathogen. Due to its saprophytic lifestyle, L . monocytogenes is assumed to infect and proliferate within soil organisms such as C aenorhabditis elegans. However, little is known about the nutrient usages and metabolite fluxes in this bacterium-nematode interaction. Here, we established a nematode colonization model for L . monocytogenes and a method for the efficient separation of the pathogen from the nematodal gut. Following 15 N labelling of C . elegans and gas chromatography-mass spectrometry-based 15 N isotopologue analysis, we detected a high basal metabolic rate of the nematode, and observed a significant metabolic flux from nitrogenous compounds of the nematode to listerial proteins during proliferation of the pathogen in the worm's intestine. For comparison, we also measured the N fluxes from the gut content into listerial proteins using completely 15 N-labelled E scherichia coli OP50 as food for C . elegans. In both settings, L . monocytogenes prefers the direct incorporation of histidine, arginine and lysine over their de novo biosynthesis. Our data suggest that colonization of nematodes is a strategy of L . monocytogenes to increase its access to N-rich nutrients.
- Subjects
EFFECT of nitrogen on microorganisms; LISTERIA monocytogenes; CAENORHABDITIS elegans; GRAM-positive bacteria; INTRACELLULAR pathogens
- Publication
Environmental Microbiology Reports, 2016, Vol 8, Issue 1, p20
- ISSN
1758-2229
- Publication type
Article
- DOI
10.1111/1758-2229.12344