We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
III.1 MOLECULAR MARKERS ASSOCIATED WITH RESISTANCE TO LEAF RUST IN BARLEY.
- Authors
MARIEY, SAMAH. A.; KHATAB, I. A.; KUMAMARU, T.
- Abstract
Genetic variation and relationship among eight barley (Hordeum vulgare L.) genotypes (four cultivars; Giza 2000, Giza 126, Giza 123 and Giza 132 and four promising lines; line 1, line 2, line 3 and line 4 were evaluated to study the marker-assisted selection associated with resistance to leaf rust (Puccinia hordei) using Sequence-Related Amplified Polymorphism (SRAP) as a new marker and first time to be reported on Egyptian barley for barley rust resistance. Results showed that the eight barley genotypes were highly different in leaf rust reaction. Data generated by nine primer combinations were sufficient to discriminate among those studied barley genotypes. Primer combinations me2+em2 had specific bands related to leaf rust resistance with band size of 1250 bp, which was found only in the resistant genotypes, while other primer combinations were found in some resistant genotypes and not found in the other susceptible ones. The (Un-weighted Pair Group Method with Arithmetic Mean) UPGMA cluster analysis of the similarity data, grouped the eight barley genotypes into two main clusters according to their reaction to leaf rust. The first cluster included the resistant genotypes (Giza 123 and Giza 132) in sub cluster and other moderately resistant genotypes (line1 and line 2) in another sub cluster, while the rest of genotypes were found in the other cluster including susceptible genotypes. These results will be useful for barley germplasm management in terms of biodiversity protection and design of new crosses for disease resistance to leaf rust breeding program.
- Subjects
MOLECULAR biology; DISEASE resistance of plants; BARLEY varieties; PLANT genetic engineering
- Publication
Egyptian Journal of Agricultural Research, 2015, Vol 93, Issue 2(B), p413
- ISSN
1110-6336
- Publication type
Article