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- Title
Comparative Measurements of Pandemic 2009 Influenza A (H1N1) Using Multiplex RT-PCR and 2 Real-Time RT-PCR Assays.
- Authors
So Young Kim; Sun Young Cho; Jin-Tae Suh; Tae Sung Park; Hee Joo Lee
- Abstract
Objective: In this study, we detected the influenza A/H1N1 virus using multiplex reverse transcription polymerase chain reaction (RT-PCR) and 2 real-time RT-PCR assays and compared these methods. Methods: Nose and throat swab samples from 48 patients with influenza-like symptoms were tested using 3 assays. Discrepant results were confirmed by sequencing. Results: The sensitivity of multiplex RT-PCR was 62.5%. Among 13 discrepant samples, 12 were concordant with real-time RT-PCR results; only 1 sample had an ambiguous result. The 2 real-time RT-PCR assays showed the same results except for 1 sample. Conclusions: The results demonstrated that concurrent real-time RT-PCR assays could be used as primary diagnostic and confirmatory assays and may provide rapid and accurate assessments of the novel H1N1 virus strain. Multiplex RT-PCR assays could detect large numbers of samples and different viral species at a time; although laboratory services using multiplex RT-PCR should not exclude the possibility of false-positive results.
- Subjects
POLYMERASE chain reaction methodology; COMPARATIVE studies; GENETIC techniques; POLYMERASE chain reaction; REVERSE transcriptase polymerase chain reaction; H1N1 influenza; INFLUENZA A virus, H1N1 subtype
- Publication
Laboratory Medicine, 2011, Vol 42, Issue 9, p558
- ISSN
0007-5027
- Publication type
Article
- DOI
10.1309/LMV4YR3EBGGCQ9PZ