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- Title
Transmission of maize chromosome 9 rearrangements in oat–maize radiation hybrids.
- Authors
Vales, M. Isabel; Riera-Lizarazu, Oscar; Rines, Howard W.; Phillips, Ronald L.; Scoles, G. J.
- Abstract
Oat–maize radiation hybrids are oat (Avena sativa L.) plants carrying radiation-induced subchromosome fragments of a given maize (Zea mays L.) chromosome. Since first-generation radiation hybrids contain various maize chromosome rearrangements in a hemizygous condition, variation might be expected in the transmission of these rearrangements to subsequent generations. The transmission and integrity of maize chromosome 9 rearrangements were evaluated in progenies of 30 oat–maize radiation hybrids by using a series of DNA-based markers and by genomic in situ hybridization. Maize chromosome 9 rearrangements were reisolated by self-fertilization in 24 of the 30 radiation hybrid lineages. Normal and deleted versions of maize chromosome 9 were transmitted at similar frequencies of 9.1% and 7.6%, respectively, while intergenomic translocations were transmitted at a significantly higher frequency of 47.6%. Most lines (93%) that inherited a rearrangement had it in the hemizygous condition. Lines with a rearrangement in the homozygous state (7%) were only identified in lineages with intergenomic translocations. Homozygous lines are more desirable from the perspective of stock maintenance, since they may stably transmit a given rearrangement to a subsequent generation. However, their isolation is not strictly required, since hemizygous lines can also be used for genome mapping studies.
- Subjects
OATS; CORN; CHROMOSOMES; CELL nuclei; GENETICS
- Publication
Genome, 2004, Vol 47, Issue 6, p1202
- ISSN
0831-2796
- Publication type
Article
- DOI
10.1139/G04-071