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- Title
Characteristics of external and internal NAD(P)H dehydrogenases in Hoya carnosa mitochondria.
- Authors
Hong, Hoang; Nose, Akihiro
- Abstract
This study aims at characterizing NAD(P)H dehydrogenases on the inside and outside of the inner membrane of mitochondria of one phospho enolpyruvate carboxykinase-crassulacean acid metabolism plant, Hoya carnosa. In crassulacean acid metabolism plants, NADH is produced by malate decarboxylation inside and outside mitochondria. The relative importance of mitochondrial alternative NADH dehydrogenases and their association was determined in intact-and alamethicin-permeabilized mitochondria of H. carnosa to discriminate between internal and external activities. The major findings in H. carnosa mitochondria are: (i) external NADPH oxidation is totally inhibited by DPI and totally dependent on Ca, (ii) external NADH oxidation is partially inhibited by DPI and mainly dependent on Ca, (iii) total NADH oxidation measured in permeabilized mitochondria is partially inhibited by rotenone and also by DPI, (iv) total NADPH oxidation measured in permeabilized mitochondria is partially dependent on Ca and totally inhibited by DPI. The results suggest that complex I, external NAD(P)H dehydrogenases, and internal NAD(P)H dehydrogenases are all linked to the electron transport chain. Also, the total measurable NAD(P)H dehydrogenases activity was less than the total measurable complex I activity, and both of these enzymes could donate their electrons not only to the cytochrome pathway but also to the alternative pathway. The finding indicated that the H. carnosa mitochondrial electron transport chain is operating in a classical way, partitioning to both Complex I and alternative Alt. NAD(P)H dehydrogenases.
- Subjects
DEHYDROGENASES; HOYA carnosa; NAD+ synthase; PLANT metabolism; PLANT mitochondria; PLANT membranes
- Publication
Journal of Bioenergetics & Biomembranes, 2012, Vol 44, Issue 6, p655
- ISSN
0145-479X
- Publication type
Article
- DOI
10.1007/s10863-012-9471-y