We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Effects of Butylated Hydroxyanisole on Ornithine Decarboxylase Activity and Its Gene Expression Induced by Phorbol Ester Tumor Promoter.
- Authors
Taniguchi, Shoji; Kono, Takeshi; Mizuno, Nobuyuki; Ishii, Masamitsu; Matsui-Yuasa, Isao; Otani, Shuzo; Hamada, Toshio
- Abstract
Butylated hydroxyanisole (BHA) is a phenolic antioxidant that has been found to suppress the activity of skin tumor promoters. In this study, we investigated the effect of BHA on the activity of ornithine decarboxylase (ODC, an indicator of tumor promotion) and its gene expression induced by 12-O-tetradecanoyl-phorbol- 13-acetate (TPA) in mouse skin. TPA-induced ODC activity was markedly inhibited by the topical application of 55 μmol of BHA (the inhibition rate at 6 h was about 80%). In Northern and dot-blot analysis, the TPA-induced increase in ODC mRNA was shown to be markedly reduced by the same dose of BHA (the inhibition rate at 4 h was about 60%). These results suggest the involvement of a decrease in ODC gene expression in the mechanism of the inhibition of ODC activity by BHA.
- Subjects
DECARBOXYLASES; PHORBOL esters; GENE expression; SKIN diseases; ANTIOXIDANTS; MESSENGER RNA
- Publication
Journal of Investigative Dermatology, 1991, Vol 96, Issue 2, p289
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1111/1523-1747.ep12464569