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- Title
B-Lymphocytes Activated by CD40 Ligand Induce an Antigen-Specific Anti-Tumour Immune Response by Direct and Indirect Activation of CD8<sup>+</sup> T-cells.
- Authors
Ritchie, D. S.; Yang, J.; Hermans, I. F.; Ronchese, F.
- Abstract
In this report, we describe the ability of CD40-ligand (CD40L)-activated, antigen-loaded B-cells to initiate antigen-specific anti-tumour immune responsesin vivo. Mice immunized by means of intravenous administration of CD40L-activated B-cells loaded with an MHC class-I-binding peptide, and challenged with a tumour cell line expressing the same class-I epitope, showed a marked delay in tumour growth, compared to non-immunized controls or to mice receiving either freshly isolated B-cells or B-cells activated with lipopolysaccharide or interleukin-4.The ability of CD40L-activated B-cells to induce antigen-specific T-cell activation appeared to be through a combination of cross-presentation of antigen from activated B-cells to resident antigen-presenting cells and direct T-cell activation by the administered B-cells themselves. Immunization with CD40L-activated B-cells may, therefore, represent a means by which to stimulate anti-tumour CD8+ T-cell responsesin vivo.
- Subjects
LYMPHOCYTES; LEUCOCYTES; KILLER cells; IMMUNOCOMPETENT cells; CELL-mediated cytotoxicity; IMMUNOLOGY; ANTIGEN-antibody reactions; CELLULAR immunity
- Publication
Scandinavian Journal of Immunology, 2004, Vol 60, Issue 6, p543
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.0300-9475.2004.01517.x