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- Title
OKT3-Induced Cytokine mRNA Expression in Human Peripheral Blood Mononuclear Cells Measured by Polymerase Chain Reaction.
- Authors
Pisa, E. K.; Pisa, P.; Hansson, M.; Wigzell, H.
- Abstract
The kinetic profile of cytokine gene expression in normal human peripheral mononuclear cells (MNC) activated by an anti-CD3 monoclonal antibody was studied. The presence or absence of 10 different cytokine mRNA were measured in a polymerase chain reaction (PCR) assisted mRNA amplification assay. After 2 h of stimulation the mRNA for interleukin-lα (IL-1α). interleukin-2 (IL-2). interleukin-3 (IL-3), tumour necrosis factor-α (TNF-α) and interferon-γ (IFN-y) were detcetable and remained present during the whole time period studied (22 h). lntcrleukin-6 (lL-6) and granulocyte macrophage colony stimulating factor (GM-CSF) were detected after 4 h. while interleukin-10 (IL-10) mRNA did not appear until after 7 h: they ail remained expressed at 22 h. A transient expression or interienkin-4 (IL-4) mRNA was observed between 4 and 7 h of stimulation. No gene expression of granulocyte colony stimulating factor (G-CSF) was detected at any time. These results show that anti-CD3 stimulation of M NC leads to a rapid sequential induction of different cytokine mRNA, some with a very transient expression.
- Subjects
MONOCLONAL antibodies; MESSENGER RNA; CELLS; POLYMERASE chain reaction; GENE expression; MACROPHAGES
- Publication
Scandinavian Journal of Immunology, 1992, Vol 36, Issue 5, p745
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1992.tb03135.x